{"id":1746,"date":"2022-10-24T20:13:52","date_gmt":"2022-10-24T20:13:52","guid":{"rendered":"http:\/\/example.com\/?p=749"},"modified":"2022-10-24T20:13:52","modified_gmt":"2022-10-24T20:13:52","slug":"749-imap-kinase-assay","status":"publish","type":"post","link":"https:\/\/w1.geleleger.nl\/rdswa-749-imap-kinase-assay-fdpkm\/","title":{"rendered":"Imap Kinase Assay"},"content":{"rendered":"<div class=\"a1420ecab7e2234e1f4e9be5cee323bb\" data-index=\"1\" style=\"float: none; margin:10px 0 10px 0; text-align:center;\">\n<script type=\"text\/javascript\">\r\n\tatOptions = {\r\n\t\t'key' : '4a1f1119f949a4af74d56b8a3af8b867',\r\n\t\t'format' : 'iframe',\r\n\t\t'height' : 250,\r\n\t\t'width' : 300,\r\n\t\t'params' : {}\r\n\t};\r\n\tdocument.write('<scr' + 'ipt type=\"text\/javascript\" src=\"http' + (location.protocol === 'https:' ? 's' : '') + ':\/\/bleedingofficecontagion.com\/4a1f1119f949a4af74d56b8a3af8b867\/invoke.js\"><\/scr' + 'ipt>');\r\n<\/script>\n<\/div>\n\n<p><strong>Imap Kinase Assay<\/strong>. <strong>This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of enzyme activity. The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. IMAP is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. Robust fluorescence signal gives reliable results with good Z factors. Diagram of the IMAP FP kinase assay.<\/strong><\/p>\n<p><figure> \t\t<img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Susan_Steinberg2\/publication\/5464493\/figure\/download\/fig5\/AS:394668937367556@1471107815348\/In-vitro-kinase-assays-showing-PKC-phosphorylation-by-Src-family-kinases-PDGFR-FAK-and.png\" alt=\"In vitro kinase assays showing PKC phosphorylation by Src family ...\" style=\"width: 100%; padding: 5px; background-color: grey;\"><figcaption>In vitro kinase assays showing PKC phosphorylation by Src family &#8230; (Madge Watson)<\/figcaption><\/figure>\n<p> \t \t<strong>Imap Kinase Assay<\/strong>. <strong>The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. The IMAP Substrate Finder Kit is an IMAP assay development tool that can significantly accelerate the identification of substrates for kinases or can be used to profile a kinase&#39;s substrate specificity. The technology is based on microfluidics on a microchip involving the electrophoretic separation and quantification of the fluorescently labeled substrate and the phosphorylated product. IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases. IMAP\u00ae is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. In other words, will the kinase activity be effectively stopped?<\/strong> Imap Kinase Assay. <\/p>\n<h3>Mobility Shift Assay (MSA)\/IMAP\u2122 Kinase Assay ALL Targets.<\/h3>\n<p>Mobility Shift Assays\/IMAP\u2122 assays directly monitor phosphorylation by measuring the non-phosphorylated and phosphorylated substrate in the kinase reaction.<\/p>\n<div style=\"display: grid;grid-template-columns: auto auto auto; background: #eee; \">\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Akiko-Murakami-Sekimata\/publication\/13368125\/figure\/fig1\/AS:601669360427009@1520460562026\/In-vitro-kinase-assay-of-the-Hrr25-T176I-and-K38A-mutant-proteins-A-hrr25-cells_Q640.jpg\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Akiko-Murakami-Sekimata\/publication\/13368125\/figure\/fig1\/AS:601669360427009@1520460562026\/In-vitro-kinase-assay-of-the-Hrr25-T176I-and-K38A-mutant-proteins-A-hrr25-cells_Q640.jpg\" onerror=\"this.onerror=null;this.src='https:\/\/tse3.mm.bing.net\/th?id=OIP.42EHAEQkJCyXm516jORdzAHaHa&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>In vitro kinase assay of the Hrr25 T176I and K38A mutant proteins. A &#8230;<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Anton_Iliuk\/publication\/224972391\/figure\/download\/fig4\/AS:340628866125836@1458223658514\/In-vitro-phosphatase-and-kinase-assays-using-pIMAGO-A-two-phosphoproteins-casein-and.png\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Anton_Iliuk\/publication\/224972391\/figure\/download\/fig4\/AS:340628866125836@1458223658514\/In-vitro-phosphatase-and-kinase-assays-using-pIMAGO-A-two-phosphoproteins-casein-and.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse2.mm.bing.net\/th?id=OIP._Xt1RwE6aJQ8x22Eucy-SAHaL-&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>In vitro phosphatase and kinase assays using pIMAGO. A, two &#8230;<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Jung-Eun_Park\/publication\/23963769\/figure\/fig1\/AS:310231759376384@1450976423215\/Development-of-a-Plk1-kinase-assay-using-GST-PBIPtide-as-a-Plk1-affinity-ligand-and-in.png\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Jung-Eun_Park\/publication\/23963769\/figure\/fig1\/AS:310231759376384@1450976423215\/Development-of-a-Plk1-kinase-assay-using-GST-PBIPtide-as-a-Plk1-affinity-ligand-and-in.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse1.mm.bing.net\/th?id=OIP.lbpwJa42O8e6FxeFW7WNvAHaFz&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>Development of a Plk1 kinase assay using GST-PBIPtide as a Plk1 &#8230;<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div style=\"display: grid;grid-template-columns: auto auto auto; background: #eee; \">\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.ddw-online.com\/media\/32\/3a-figure-23.jpg\"><img decoding=\"async\" src=\"https:\/\/www.ddw-online.com\/media\/32\/3a-figure-23.jpg\" onerror=\"this.onerror=null;this.src='https:\/\/tse1.mm.bing.net\/th?id=OIP.fdthcA2AKlfm0UhMyYI0kQHaHW&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>TR-FRET Based Assays &#8211; Getting better with age &#8211; Drug Discovery World (DDW)<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.moleculardevices.co.jp\/wp-content\/uploads\/2017\/10\/IMAP-Tech-PDE-TRF_principle.gif\"><img decoding=\"async\" src=\"https:\/\/www.moleculardevices.co.jp\/wp-content\/uploads\/2017\/10\/IMAP-Tech-PDE-TRF_principle.gif\" onerror=\"this.onerror=null;this.src='https:\/\/tse4.mm.bing.net\/th?id=OIP.rjaBuY84onJZOIqKEONphgHaEn&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>Enzyme &#8211; IMAP Assays &#8211; \u30e2\u30ec\u30ad\u30e5\u30e9\u30fc\u30c7\u30d0\u30a4\u30b9\u30b8\u30e3\u30d1\u30f3\u682a\u5f0f\u4f1a\u793e<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Andrew-Ferguson-17\/publication\/49624684\/figure\/fig4\/AS:648993461772288@1531743507068\/The-binding-site-of-human-CK2a-in-complex-with-AMPPNP-The-AMPPNP-molecule-is-shown-as_Q320.jpg\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Andrew-Ferguson-17\/publication\/49624684\/figure\/fig4\/AS:648993461772288@1531743507068\/The-binding-site-of-human-CK2a-in-complex-with-AMPPNP-The-AMPPNP-molecule-is-shown-as_Q320.jpg\" onerror=\"this.onerror=null;this.src='https:\/\/tse2.mm.bing.net\/th?id=OIP.htkVAzLvw6WcDoeDcGN-4gAAAA&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>(a) Chemical structure of CX-4945. (b) IMAP assay in which kinase &#8230;<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div style=\"display: grid;grid-template-columns: auto auto auto; background: #eee; \">\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/publication\/320391877\/figure\/fig2\/AS:550957491474433@1508369910296\/Two-step-AlphaScreen-kinase-assay-a-Schematic-assay-presentation-b-AMPK-is-inactive_Q640.jpg\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/publication\/320391877\/figure\/fig2\/AS:550957491474433@1508369910296\/Two-step-AlphaScreen-kinase-assay-a-Schematic-assay-presentation-b-AMPK-is-inactive_Q640.jpg\" onerror=\"this.onerror=null;this.src='https:\/\/tse1.mm.bing.net\/th?id=OIP.5dITtNYmGHzHZJqWgu3AvAHaHa&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>One-step AlphaScreen kinase assay. (a) Schematic presentation of the &#8230;<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/kinaselogistics.com\/fileadmin\/user_upload\/profiling\/adp-glo.png\"><img decoding=\"async\" src=\"https:\/\/kinaselogistics.com\/fileadmin\/user_upload\/profiling\/adp-glo.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse2.mm.bing.net\/th?id=OIP.N0pUxJmwZJNGnE088Fn6uAHaKl&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>Activity-based Kinase Profiling Service | Kinase Logistics: kinaselogistics<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"http:\/\/www.carnabio.com\/common\/images\/product\/tree-mapk.png\"><img decoding=\"async\" src=\"http:\/\/www.carnabio.com\/common\/images\/product\/tree-mapk.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse2.mm.bing.net\/th?id=OIP.imZmBYXUeuY22yX-pj30ugHaHa&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>Mobility Shift Assay(MSA)\/IMAP\u2122 Kinase Assay QuickScout\u2122 MAPK Cascade &#8230;<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<div style=\"display: grid;grid-template-columns: auto auto auto; background: #eee; \">\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/publication\/308663729\/figure\/download\/fig4\/AS:410923257876484@1474983147451\/n-vitro-kinase-assay-against-candidate-proteins-a-Histone-H1-kinase-assay-Proteins-were.png\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/publication\/308663729\/figure\/download\/fig4\/AS:410923257876484@1474983147451\/n-vitro-kinase-assay-against-candidate-proteins-a-Histone-H1-kinase-assay-Proteins-were.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse2.mm.bing.net\/th?id=OIP.oB7fdKOArK9itQX-OE1QOwHaCS&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>In vitro kinase assay against candidate proteins. a Histone H1 kinase &#8230;<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Said_Goueli2\/publication\/13934342\/figure\/download\/fig2\/AS:385622012383232@1468950860063\/A-and-B-immunoprecipitation-kinase-assay-for-ERK-activity-in-K562-cells-treated-with.png\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Said_Goueli2\/publication\/13934342\/figure\/download\/fig2\/AS:385622012383232@1468950860063\/A-and-B-immunoprecipitation-kinase-assay-for-ERK-activity-in-K562-cells-treated-with.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse3.mm.bing.net\/th?id=OIP.TWugwE2Hwsb5uRbGqResnwHaH3&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>A and B, immunoprecipitation kinase assay for ERK activity in K562 &#8230;<\/p>\n<\/p><\/div>\n<div style=\"padding: 1em;\"> \t\t        <a href=\"https:\/\/www.researchgate.net\/profile\/Albert_Van_Geelen2\/publication\/6689812\/figure\/download\/fig3\/AS:281505734643717@1444127605586\/In-vitro-protein-kinase-assay-A-Viral-substrates-Sub-strates-and-the-protein-kinases.png\"><img decoding=\"async\" src=\"https:\/\/www.researchgate.net\/profile\/Albert_Van_Geelen2\/publication\/6689812\/figure\/download\/fig3\/AS:281505734643717@1444127605586\/In-vitro-protein-kinase-assay-A-Viral-substrates-Sub-strates-and-the-protein-kinases.png\" onerror=\"this.onerror=null;this.src='https:\/\/tse3.mm.bing.net\/th?id=OIP.ufoOipWeFNHhwcetWVH16QHaHc&amp;pid=Api';\" style=\"width:100%;margin-top: 8px;vertical-align: middle;\"><\/a> \t\t        <\/p>\n<p>In vitro protein kinase assay. (A) Viral substrates. Sub- strates and &#8230;<\/p>\n<\/p><\/div>\n<\/p><\/div>\n<p><strong>Imap Kinase Assay<\/strong>. <strong>Protein kinases are one of the largest families of evolutionarily related proteins and comprise one of the most abundant gene families in humans. The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of enzyme activity. Available in both FP and TR-FRET detection modes. The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. The assay is a simple &#34;mix-and-read&#34; procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. Diagram of the IMAP FP kinase assay.<\/p>\n<p><\/strong> Imap Kinase Assay.<\/p>\n<div class=\"a1420ecab7e2234e1f4e9be5cee323bb\" data-index=\"2\" style=\"float: none; margin:10px 0 10px 0; text-align:center;\">\n<script type=\"text\/javascript\">\r\n\tatOptions = {\r\n\t\t'key' : '4a1f1119f949a4af74d56b8a3af8b867',\r\n\t\t'format' : 'iframe',\r\n\t\t'height' : 250,\r\n\t\t'width' : 300,\r\n\t\t'params' : {}\r\n\t};\r\n\tdocument.write('<scr' + 'ipt type=\"text\/javascript\" src=\"http' + (location.protocol === 'https:' ? 's' : '') + ':\/\/bleedingofficecontagion.com\/4a1f1119f949a4af74d56b8a3af8b867\/invoke.js\"><\/scr' + 'ipt>');\r\n<\/script>\n<\/div>\n\n<div style=\"font-size: 0px; height: 0px; line-height: 0px; margin: 0; padding: 0; clear: both;\"><\/div>","protected":false},"excerpt":{"rendered":"<p>Imap Kinase Assay. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"_links":{"self":[{"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/posts\/1746"}],"collection":[{"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/comments?post=1746"}],"version-history":[{"count":0,"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/posts\/1746\/revisions"}],"wp:attachment":[{"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/media?parent=1746"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/categories?post=1746"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/w1.geleleger.nl\/wp-json\/wp\/v2\/tags?post=1746"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}