Imap Kinase Assay. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. The assay is a simple "mix-and-read" procedure that allows accurate determination of enzyme activity. The assay is a simple "mix-and-read" procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. IMAP is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. Robust fluorescence signal gives reliable results with good Z factors. Diagram of the IMAP FP kinase assay.
Imap Kinase Assay. The assay is a simple "mix-and-read" procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. The IMAP Substrate Finder Kit is an IMAP assay development tool that can significantly accelerate the identification of substrates for kinases or can be used to profile a kinase's substrate specificity. The technology is based on microfluidics on a microchip involving the electrophoretic separation and quantification of the fluorescently labeled substrate and the phosphorylated product. IMAP provides a complete assay system for screening kinases, phosphatases, and phosphodiesterases. IMAP® is a fluorescence polarisation-based assay method which can be applied to the measurement of protein kinase activity. In other words, will the kinase activity be effectively stopped? Imap Kinase Assay.
Mobility Shift Assay (MSA)/IMAP™ Kinase Assay ALL Targets.
Mobility Shift Assays/IMAP™ assays directly monitor phosphorylation by measuring the non-phosphorylated and phosphorylated substrate in the kinase reaction.
Imap Kinase Assay. Protein kinases are one of the largest families of evolutionarily related proteins and comprise one of the most abundant gene families in humans. The assay is a simple "mix-and-read" procedure that allows accurate determination of enzyme activity. Available in both FP and TR-FRET detection modes. The assay is a simple "mix-and-read" procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. The assay is a simple "mix-and-read" procedure that allows accurate determination of kinase, phosphatase, and phosphodiesterase (PDE) activity. Diagram of the IMAP FP kinase assay.
Imap Kinase Assay.